Syftet med denna uppsats har varit att beskriva ett tillvägagångssätt för att hantera den alltmer utbredda sjukdomen kastanjeblödarsjuka. Kastanjeblödarsjuka är en relativt ny sjukdom som drabbar hästkastanjer, Aesculus hippocastanum. Den upptäcktes i Holland så sent som år 2002 och nyligen har även Sverige drabbats. Sjukdomen orsakas av bakterien Pseudomonas syringae pv. aesculi. I många europeiska parker och alléer har blödande hästkastanjer blivit ett stort problem men även platser i vårt närområde har drabbats, som t.ex.

Syfte: Syftet med denna studie var att jämföra antibiotikabehandling med corneal collagen crosslinking (CXL)-behandling genom fotoaktivering av riboflavin vid bakteriell keratit. Att experimentellt undersöka om en viss stam av Pseudomonas aeruginosa kunde infektera cornealt epitel var också en del av studien.Metod: Litteraturstudie samt ett experiment med Pseudomonas aeruginosa och grisögon in vitro.Resultat: Dagens behandling av keratit är initial med antibiotika. I experimentella studier har CXL använts både i kombination med antibiotika och som enskild behandling. Positiva resultat från studier med CXL som behandling mot keratit har rapporterats.Resultatet av det experimentella försöket pekar på att den stam av Pseudomonas aeruginosa som användes i experimentet inte kan penetrera ett intakt epitel.Slutsats: CXL kan bli en viktig och användbar behandlingsform vid keratit, särskilt med tanke på den ökade antibiotikaresistensutvecklingen. Ytterligare forskning på området krävs dock innan metoden kan bli fullt vedertagen.För att kunna fastställa om stammen av Pseudomonas aeruginosa i det aktuella experimentet har virulensfaktorer för att ta sig in i ett oskadat epitel eller inte krävs fler försök..

Fusarium graminearum causes reduction in both yield and quality in cereal grain worldwide. One of the diseases it can cause is Fusarium Head Blight (FHB). Both sexually and asexually produced spores can infect living plants. Asexual conidia are produced in the mycelium while sexual ascospores form in asci in perithecia on debris. In biological control, living organsims are used to control pests and diseases.

Canine otitis externa is a common reason for a visit to the vet. Predisposing factors often complicate treatment and may result in a chronic disease progression. All otic preparation for topical use in Fass Vet contain antibiotics, corticosteroids and a fungicide. Sixty cerumen samples from dogs with external otitis, sent to the Department of Bacteriology at SVA, were categorised according to their profile of isolated microorganisms. Staphylococcus intermedius alone or together with Malassezia pachydermatis were the most common organisms found. Fifteen percent of the samples contained M.

etta arbete belyser det för hästnäringen stora problemet ekvina placentiter från en veterinärmedicinsk synvinkel. Ekvin placentit betyder moderkaksinflammation hos häst. De vanligaste orsakande virusen, EHV-1 och EVA, samt ett mykotiskt agens, Histoplasma, tas upp. Bakteriella placentiter är dock mycket oftare förekommande. Därför diskuteras inte bara de tre agens som är vanligast; Streptococcus zooepidemicus, Escherichia coli och Pseudomonas aeruginosa, utan även två bakteriella agens på snabb frammarsch; Leptospira spp.

etta arbete belyser det för hästnäringen stora problemet ekvina placentiter från en veterinärmedicinsk synvinkel. Ekvin placentit betyder moderkaksinflammation hos häst. De vanligaste orsakande virusen, EHV-1 och EVA, samt ett mykotiskt agens, Histoplasma, tas upp. Bakteriella placentiter är dock mycket oftare förekommande. Därför diskuteras inte bara de tre agens som är vanligast; Streptococcus zooepidemicus, Escherichia coli och Pseudomonas aeruginosa, utan även två bakteriella agens på snabb frammarsch; Leptospira spp.

Det här arbetet handlar om vilka mikrobiologiska faktorer som kan påverkar snittblommors livslängd. Koncentrationen av mikroorganismer i vasvattnet har en större betydelse för livslängden, mer än vilken sort av mikroorganismer som förekommer i vasvattnet. I vasvattnet till snittblommor har man funnit bland annat Pseudomonas, Bacillus och Enterobacter. För att minska påverkan på snittblommorna av dessa arter så bör habitatet för dessa arter ändras för att missgynna dem och därmed indirekt gynna snittblommorna. Arbetet tar även upp andra faktorer som kan påverka livslängden och prydnadsvärdet, så som etylen.

The tomato is an important crop worldwide, and in recent years it has received great attention due to its rich content of carotenoids, which in studies indicate to have health benefiting properties. Many studies have been done in this area of research and in how to optimize the carotenoid content in tomatoes and tomato products. The objectives of this essay were to summarize present data in this field. The results were as follows:There are nine different carotenoids identified in tomatoes. Lycopene and ?-carotene are the ones that have been given the greatest attention.

I Sverige drabbas cirka 10 % av alla patienter som vårdas inom sjukvården av vårdrelaterade infektioner. För att minimera riskerna för både patienter och personal läggs stor vikt vid vårdhygien, i syftet att minska förekomsten av smittoämnen. Vårdhygien tros även vara en avgörande faktor för att begränsa den globala spridningen av antibiotikaresistenta bakterier.Syftet med studien var att undersöka om ett ytdesinfektionsmedel med kvardröjande effekt kunde leda till signifikant minskning av förekomsten av bakterier i en aktiv vårdmiljö jämfört med det rutinmässigt använda ytdesinfektionsmedlet. Båda ytdesinfektionsmedlen var isopropanolbaserade. Prover togs från 14 tagytor i enkelrum med sluss som städats med respektive medel på en vårdavdelning.

The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..